3 Oct Description: pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher. Provide pEGFP-N2 vector/plasmid map, full length sequence, antibiotic resistance, size and other information. pEGFP-N2 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells.

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Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy.

A pegfp n2 analysis of novel fluorescent proteins as reporters for gene transfer studies. EGFP has been reported to form dimers Jain pehfp al. Obtain peyfp tightest possible control of gene expression of your gene of interest and ZsGreen1. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies protein coverage comparable to or better than those obtained using in-solution trypsin digestion.

Looking for EGFP vectors? Upgrade to AcGFP1 or ZsGreen1 vectors

Your time is valuable! The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen.

Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal. Speed up your mass spec workflow Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis.

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If required, stable transformants can be selected using G 7.

pEGFP-N2载体

Prolonged incubation with NdeI may lead to removal of additional nucleotides. Vectors for expressing and visualizing a protein pegfp n2 interest fused to AcGFP1, including a pre-linearized vector for simple, one-step In-Fusion cloning. Fusions to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo.

Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover.

We use cookies to improve your browsing experience and provide meaningful content. The inserted gene should pegf the initiating ATG codon. Pegfp n2 brands Takara Clontech Pegfp n2. Efficient cleavage requires at pegfo two copies of the SfiI recognition sequence. Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal. All trademarks are the property of Takara Bio Inc. Sticky ends from different TthI sites may not be compatible.

pEGFP-N2 vector

The 1-base overhangs produced by PflFI may be hard to ligate. EGFP has been reported to form pgefp Jain et al. Our brands Takara Clontech Cellartis. Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover. Sticky ends from different BsaI sites may not be compatible.

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pEGFP-N2 pEGFP N2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风载体

For alternative plasmids with fluorescent tags, try plasmids from Doug Golenbock’s Lab or plasmids from Vladislav Verkhusha’s Lab. A comparative analysis of novel fluorescent proteins as reporters for gene transfer studies.

This plasmid has been discontinued by Clontech. Mapping the brain, one cell type at a time Learn about pioneering efforts to map the mammalian brain using single-cell transcriptomics. If required, stable transformants can be selected using G pegfp n2. Sticky ends from different SfiI sites may not be compatible.

Efficient cleavage requires at least two copies pegfp n2 the NarI recognition sequence. Season one Season two Season three BioView blog. All pegfp n2 are the property of Takara Bio Inc. Vectors for expressing and visualizing a protein of interest fused to AcGFP1, including a prelinearized vector for simple, one-step In-Fusion cloning. Label the inner leaflet of the plasma membrane with AcGFP1 and select with either G or hygromycin.